A radioimmunoassay for tauro-beta-muricholic acid suitable for use with isolated rat liver cells
- PMID: 6825837
- DOI: 10.1016/0014-5793(83)80333-0
A radioimmunoassay for tauro-beta-muricholic acid suitable for use with isolated rat liver cells
Abstract
A new radioimmunoassay which can be used to measure the amounts of tauro-beta-muricholic acid produced by isolated rat hepatocytes in vitro is described. Cross reactivities of other bile acids known to be present in rat liver with the antiserum used in the assay were not sufficient to interfere with the measurement of tauro-beta-muricholic acid. Exogenous taurochenodeoxycholic acid was metabolised by isolated rat hepatocytes concurrently with the appearance of tauro-beta-muricholic acid in the cell.
Similar articles
-
The metabolism of chenodeoxycholic acid to beta-muricholic acid in rat liver.Eur J Biochem. 1983 Jul 15;134(1):191-6. doi: 10.1111/j.1432-1033.1983.tb07550.x. Eur J Biochem. 1983. PMID: 6861760
-
Tauroursodeoxycholate and tauro-beta-muricholate exert cytoprotection by reducing intrahepatocyte taurochenodeoxycholate content.Hepatology. 1993 Mar;17(3):470-6. Hepatology. 1993. PMID: 8383089
-
A radioimmunoassay of bile acids.J Immunoassay. 1990;11(3):355-72. doi: 10.1080/01971529008055038. J Immunoassay. 1990. PMID: 2229424
-
The effect of dietary fat on bile salt synthesis in rat liver.Biochim Biophys Acta. 1983 Jul 12;752(2):307-14. doi: 10.1016/0005-2760(83)90128-5. Biochim Biophys Acta. 1983. PMID: 6860705
-
Measurement of conjugated 1 beta-hydroxycholic acid in urine of newborns by specific radioimmunoassay.Chem Pharm Bull (Tokyo). 1992 Mar;40(3):701-4. doi: 10.1248/cpb.40.701. Chem Pharm Bull (Tokyo). 1992. PMID: 1611682
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources