doi: 10.1016/0027-5107(83)90081-7.
SCE induction is proportional to substitution in DNA for thymidine by CldU and BrdU
- PMID: 6828032
- DOI: 10.1016/0027-5107(83)90081-7
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SCE induction is proportional to substitution in DNA for thymidine by CldU and BrdU
Mutat Res.
1983 Jan.
Abstract
The incorporation of 5-bromo- and 5-chlorodeoxyuridine into cellular DNA and the induction of sister-chromatid exchanges (SCE) was examined with Chinese hamster ovary cells in culture. Although CldU caused 3-5 times more SCE than BrdU at equal extracellular concentrations, the 2 analogs were incorporated into DNA in place of thymidine similarly over a concentration range of 0.5-20 microM. The results show that SCE induction is linearly proportional to substitution for thymidine by both BrdU and CldU. Finally, based on linear extrapolation, a spontaneous level of approximately 6 SCEs per cell is estimated.
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