Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Jan 25;11(2):477-88.
doi: 10.1093/nar/11.2.477.

Solid phase synthesis of polynucleotides. VIII. Synthesis of mixed oligodeoxyribonucleotides by the phosphotriester solid phase method

Y Ike, S Ikuta, M Sato, T Huang, K Itakura
Free PMC article

Solid phase synthesis of polynucleotides. VIII. Synthesis of mixed oligodeoxyribonucleotides by the phosphotriester solid phase method

Y Ike et al. Nucleic Acids Res. .
Free PMC article

Abstract

A solid phase method for the simultaneous synthesis of mixed oligonucleotides using a phosphotriester approach has been developed. For this synthesis, a mixture of mono or dimeric coupling units is used, and a slight difference in the reactivity of those units is found. However, this difference does not hamper the simultaneous, mixed oligonucleotide synthesis, and the sequence analysis of a product demonstrates the existence of all desired sequences in the final mixture.

PubMed Disclaimer

References

    1. Biochemistry. 1978 Apr 4;17(7):1257-67 - PubMed
    1. Methods Enzymol. 1980;65(1):620-38 - PubMed
    1. Nucleic Acids Res. 1981 Feb 25;9(4):879-94 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6613-7 - PubMed
    1. Immunogenetics. 1981;14(5):383-92 - PubMed

Publication types

MeSH terms