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. 1983 Feb 11;272(2):351-8.
doi: 10.1016/s0378-4347(00)86138-1.

Determination of tamoxifen and metabolites in human serum by high-performance liquid chromatography with post-column fluorescence activation

R R Brown, R Bain, V C Jordan

Determination of tamoxifen and metabolites in human serum by high-performance liquid chromatography with post-column fluorescence activation

R R Brown et al. J Chromatogr. .

Abstract

Sensitive and reproducible analyses were developed for assaying tamoxifen, monohydroxytamoxifen, N-desmethyltamoxifen, metabolite E [trans-1(4-hydroxyphenyl)1,2-diphenylbut-1-ene] and a new metabolite, metabolite Y [trans-1(4-hydroxyethoxyphenyl)-1,2-diphenylbut-1-ene] in human serum using high-performance liquid chromatography (HPLC). Three different systems were developed for specific purposes. All chromatography was performed using serum extracts made with hexane-butanol. Detection was by fluorimetry of phenanthrene derivatives formed by on-stream UV irradiation with a newly described device for post-column irradiation of the HPLC stream. This device may be of use in other HPLC systems requiring post-column photochemical reactions.

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