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. 1983 Mar 16;111(2):444-9.
doi: 10.1016/0006-291x(83)90326-1.

Photolabeling of staphylococcal alpha-toxin from within rabbit erythrocyte membranes

M Thelestam, C Jolivet-Reynaud, J E Alouf

Photolabeling of staphylococcal alpha-toxin from within rabbit erythrocyte membranes

M Thelestam et al. Biochem Biophys Res Commun. .

Abstract

Intrinsic membrane proteins of rabbit red blood cells were labeled with the photoreactive amphipatic reagent 12-(4-azido-2-nitrophenoxy) stearoyl (1-14C) glucosamine, which inserts into the hydrophobic membrane region and generates a reactive nitrene upon ultraviolet irradiation. Photolabeling of membrane-bound staphylococcal alpha-toxin after lysis of probe-treated rabbit red blood cells by this toxin implies its penetration into the hydrophobic region of the outer leaflet of the membrane. In contrast clostridial theta-toxin and staphylococcal delta-toxin were not labeled, but extraction of intrinsic membrane proteins by delta-toxin was evidenced.

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