Induced segregation of human syntenic genes by 5-bromodeozyuridine + near-visible light
- PMID: 684558
- DOI: 10.1007/BF01538867
Induced segregation of human syntenic genes by 5-bromodeozyuridine + near-visible light
Abstract
Chromosome-breaking agents have been used in two different procedures for promoting segregation of syntenic genes on human chromosome 12. In method A, a human-Chinese hamster cell hybrid containing the single human chromosome 12 was treated either with 5-bromodeoxyuridine BrdU + near-visible light or with X-rays. In method B, normal human fibroblasts were treated with BrdU + near-visible light followed by their fusion with a Chinese hamster glycine-requiring cell mutant CHO-K1/gly-A. Since the human complementing gene for serine hydroxymethyltransferase, an enzyme deficient in gly-A, lies on human chromosome 12, only those hybrids retaining that chromosome can survive the glycine-free medium. Clones isolated from both procedures were analyzed for the loss or retention of four other syntenic genes on chromosome 12, TPI, GAPD, LDH B, and PepB. The results demonstrate that method B is much more effective in generating clones with extensive marker losses. In addition, the segregation pattern and frequency obtained in this study provided information on the linear order of TPI and GAPD on chromosome 12.
Similar articles
-
Regional assignment of human genes TPI1, GAPDH, LDHB, SHMT, and PEPB on chromosome 12.Cytogenet Cell Genet. 1979;24(2):102-14. doi: 10.1159/000131363. Cytogenet Cell Genet. 1979. PMID: 477403
-
Chromosomal assignment and regional localization of CS, ENO2, GAPDH, LDHB, PEPB, and TPI in man-rodent cell hybrids.Cytogenet Cell Genet. 1978;22(1-6):482-6. doi: 10.1159/000131003. Cytogenet Cell Genet. 1978. PMID: 318158 No abstract available.
-
Human glyceraldehyde-3-phosphate dehydrogenase in man-rodent somatic cell hybrids.Science. 1976 Apr 2;192(4234):54-6. doi: 10.1126/science.176725. Science. 1976. PMID: 176725
-
Expression of GAPDH and TPI in dog-rodent hybrids.Cytogenet Cell Genet. 1978;22(1-6):547-51. doi: 10.1159/000131021. Cytogenet Cell Genet. 1978. PMID: 752540 No abstract available.
-
Assignment of the genes for triose phosphate isomerase to chromosome 6 and tripeptidase-1 to chromosome 10 in Mus musculus by somatic cell hybridization.Somatic Cell Genet. 1978 Mar;4(2):233-40. doi: 10.1007/BF01538987. Somatic Cell Genet. 1978. PMID: 566475
Cited by
-
Isolation and localization of DNA segments from specific human chromosomes.Proc Natl Acad Sci U S A. 1980 May;77(5):2829-33. doi: 10.1073/pnas.77.5.2829. Proc Natl Acad Sci U S A. 1980. PMID: 6930670 Free PMC article.
-
Isolation of a human repetitive sequence and its application to regional chromosome mapping.Proc Natl Acad Sci U S A. 1982 Dec;79(23):7390-4. doi: 10.1073/pnas.79.23.7390. Proc Natl Acad Sci U S A. 1982. PMID: 6961418 Free PMC article.
-
Isolation and chromosomal localization of unique DNA sequences from a human genomic library.Proc Natl Acad Sci U S A. 1982 Feb;79(3):865-9. doi: 10.1073/pnas.79.3.865. Proc Natl Acad Sci U S A. 1982. PMID: 7038688 Free PMC article.
-
Regional mapping of the gene for human lysosomal acid phosphatase (ACP2) using a hybrid clone panel containing segments of human chromosome 11.Hum Genet. 1978 Nov 24;45(1):1-10. doi: 10.1007/BF00277567. Hum Genet. 1978. PMID: 730175
-
Regional mapping of the phenylalanine hydroxylase gene and the phenylketonuria locus in the human genome.Proc Natl Acad Sci U S A. 1985 Sep;82(18):6221-5. doi: 10.1073/pnas.82.18.6221. Proc Natl Acad Sci U S A. 1985. PMID: 3862128 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Research Materials
Miscellaneous