Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Jan;128(1):181-5.
doi: 10.1016/0003-2697(83)90360-3.

An NADH-coupled assay for femtogram or nanogram quantities of chymotrypsin

E A Mroz, C Lechene

An NADH-coupled assay for femtogram or nanogram quantities of chymotrypsin

E A Mroz et al. Anal Biochem. 1983 Jan.

Abstract

Chymotrypsin can be determined with an NADH-coupled assay. Hydrolysis of the substrate benzoyltyrosine ethyl ester is monitored by coupling the liberation of ethanol to the production of NADH and determining the NADH spectrophotometrically or fluorometrically. Nanogram quantities of chymotrypsin can be determined in milliliter volumes. With these microfluorescence methods this assay can be performed in a final volume of less than a nanoliter, allowing determination of femtogram quantities of chymotrypsin, the amount present in an individual zymogen granule.

PubMed Disclaimer

Publication types

LinkOut - more resources