Kinetic mechanism of the molecular forms of chicken liver mitochondrial malate dehydrogenase

Abstract

1. The reaction kinetic mechanism (pH 7.4) of the molecular forms of chicken liver m-MDH is of the order bi-bi ternary complex type with the existence of the E-oxaloacetate, E-L-malate, E-NAD+-oxaloacetate, E-NADH-L-malate, E-NAD+-NADH, E-NAD+-NAD+, E-NADH-NAD+ and E-NAD-NADH abortive complexes. 2. The saturating concentration values of the substrates are notably modified, in certain cases, in the presence of the reaction products.