doi: 10.1093/nar/11.10.3393.
Specifically alkylated DNA fragments. Synthesis and physical characterization of d[CGC(O6Me)GCG] and d[CGT(O6Me)GCG]
- PMID: 6856460
- PMCID: PMC325971
- DOI: 10.1093/nar/11.10.3393
Item in Clipboard
Specifically alkylated DNA fragments. Synthesis and physical characterization of d[CGC(O6Me)GCG] and d[CGT(O6Me)GCG]
Nucleic Acids Res.
.
Free PMC article
Abstract
Two hexamer DNA fragments containing a carcinogenic modified base, O6-methyl guanine, have been synthesized by a solid-phase phosphotriester method, in which the unmodified guanine residues present were O6 protected with the 4-nitrophenylethyl group. These two alkylated oligonucleotides were found to have similar Tm's about 40 degrees lower than the unmodified parent compound, d(CG)3. Moreover, the presence of the (O6Me)G appears to inhibit the B leads to Z transition, as determined by CD spectroscopy.
Similar articles
-
Synthesis and characterization of a set of four dodecadeoxyribonucleoside undecaphosphates containing O6-methylguanine opposite adenine, cytosine, guanine, and thymine.Biochemistry. 1984 Nov 20;23(24):5686-91. doi: 10.1021/bi00319a004. Biochemistry. 1984. PMID: 6525333
-
Synthesis and physical characterization of the self-complementary, alternating pyrimidine/purine hexanucleotide d[CGTACG].Nucleic Acids Res. 1982 Oct 25;10(20):6265-71. doi: 10.1093/nar/10.20.6265. Nucleic Acids Res. 1982. PMID: 7177847 Free PMC article.
-
Crystal and molecular structure of a DNA duplex containing the carcinogenic lesion O6-methylguanine.Biochemistry. 1990 Nov 20;29(46):10461-5. doi: 10.1021/bi00498a005. Biochemistry. 1990. PMID: 2271657
-
The influence of the purine 2-amino group on DNA conformation and stability. Synthesis and conformational analysis of d[T(2-aminoA)]3.Nucleic Acids Res. 1982 Jul 24;10(14):4351-61. doi: 10.1093/nar/10.14.4351. Nucleic Acids Res. 1982. PMID: 7122239 Free PMC article.
-
Crystal structure of a mispaired dodecamer, d(CGAGAATTC(O6Me)GCG)2, containing a carcinogenic O6-methylguanine.Biochemistry. 1994 Mar 29;33(12):3487-93. doi: 10.1021/bi00178a004. Biochemistry. 1994. PMID: 8142345
Cited by
-
High-resolution structure of a mutagenic lesion in DNA.Proc Natl Acad Sci U S A. 1990 Dec;87(24):9573-6. doi: 10.1073/pnas.87.24.9573. Proc Natl Acad Sci U S A. 1990. PMID: 2263612 Free PMC article.
-
B- to Z-DNA transition probed by oligonucleotides containing methylphosphonates.Proc Natl Acad Sci U S A. 1986 Mar;83(6):1617-21. doi: 10.1073/pnas.83.6.1617. Proc Natl Acad Sci U S A. 1986. PMID: 3456606 Free PMC article.
-
Use of oligonucleotides containing ethenoadenine to study the repair of this DNA lesion. Determination of individual and collective repair activities.Arch Toxicol. 1994;68(6):358-63. doi: 10.1007/s002040050082. Arch Toxicol. 1994. PMID: 8092927
-
DNA stability in the gas versus solution phases: a systematic study of thirty-one duplexes with varying length, sequence, and charge level.J Am Soc Mass Spectrom. 2006 Oct;17(10):1383-1395. doi: 10.1016/j.jasms.2006.07.016. Epub 2006 Aug 17. J Am Soc Mass Spectrom. 2006. PMID: 16914323
-
Guanine modification during chemical DNA synthesis.Nucleic Acids Res. 1987 Oct 26;15(20):8333-49. doi: 10.1093/nar/15.20.8333. Nucleic Acids Res. 1987. PMID: 3671086 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
