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. 1983 May 11;11(9):2575-84.
doi: 10.1093/nar/11.9.2575.

Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides

T Dörper, E L Winnacker
Free PMC article

Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides

T Dörper et al. Nucleic Acids Res. .
Free PMC article

Abstract

The paper describes an improved method for the synthesis of oligodeoxyribonucleotides using phosphoramidite chemistry. Our procedure relies on novel phosphoramidite intermediates, the deoxyribonucleoside-3'-morpholine-methoxyphosphins. These compounds are extremely stable and can be purified readily. Condensation reactions during solid-phase synthesis can thus be performed with high efficiency and result in a high yield synthesis of long chain oligodeoxyribonucleotides.

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