Pulmonary mechanics in conscious Fischer 344 rats: multiple evaluations using nonsurgical techniques

Abstract

An adaptation of the method reported by Amdur and Mead (1958, Amer. J. Physiol. 192, 364-368) was used to evaluate pulmonary mechanics in conscious rats, utilizing a flow plethysmograph and intraesophageal catheter to determine compliance, resistance, respiratory flow, thoracic pressure, tidal volume, frequency, and minute volume. The procedure did not require surgery, and repeat evaluations were made in conscious rats. Real time calculations of pulmonary parameters were accomplished with an analog computer interfaced with a digital computer for display and data storage. Multiple evaluations (1, 3, 6, 13, 18, 24, 30, and 36 weeks) in 40 rats are reported. The utility of the system in establishing a base line against which pulmonary impairment, related to the effects of known pulmonary toxicants, e.g., ANTU and NO2, could be measured, was evaluated.