Mechanical properties of rat cerebral arteries as studied by a sensitive device for recording of mechanical activity in isolated small blood vessels

Abstract

A sensitive device for recording of mechanical activity in isolated small blood vessels with calibres down to 100 microns is described. This equipment was used to examine the mechanical properties of rat cerebral arteries. The ultrastructure of the preparations was investigated by light-, transmission, and scanning electron-microscopy. In general the walls of the middle cerebral and basilar arteries consisted of 3 layers of smooth muscle cells, which occupied approximately 80% of the total wall thickness. The present technique preserved the integrity of the vessel wall and caused no observable damage to the smooth muscle or endothelial cells. Neither the basilar nor the middle cerebral arteries developed spontaneous phasic contractions under standard conditions. Potassium excess (124 mM) induced a biphasic contractile response characterized by a fast and partly transient increase in tension (phase A), followed by a slowly developing sustained contraction (phase B). The responses to K+ were strong, highly reproducible and not influenced by pH changes in the range 6.9-7.8, making K+-stimulation suitable for testing of vascular contractility. Length-tension measurements were performed on relaxed and K+-activated basilar arteries. The mechanical behaviour of the vessels conformed to a sliding-filament model of muscular contraction. Using the "Maxwell model" of a muscle, the length at which the contractile element produced maximum active tension was established. The passive wall tension at this length (approximately 1 mN/mm) averaged only about 20% of the total wall tension the arteries were capable of producing when activated by K+. Under isometric conditions the K+-contracted basilar artery developed a maximum active wall stress of approximately 240 mN/mm2. In the light of the mechanical data obtained from the length-tension measurements, the optimum resting wall tension for registration of vascular responses is discussed. It appears that the present in vitro system can be of great value in investigations of the smooth muscle function in small blood vessels.