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. 1983 Jul 5;758(1):30-6.
doi: 10.1016/0304-4165(83)90006-5.

Characterization of oligosaccharide units of p-N-collagen type III from dermatosparactic bovine skin

H Shinkai, C M Lapiere

Characterization of oligosaccharide units of p-N-collagen type III from dermatosparactic bovine skin

H Shinkai et al. Biochim Biophys Acta. .

Abstract

p-N-collagen type III was extracted from dermatosparactic and normal fetal bovine skin and purified by ion-exchange chromatography using DEAE- and CM-cellulose. Asparagine-linked sugar chains were fractionated by high voltage paper chromatography from the products obtained after hydrazinolysis and reduction with NaB3H4. These oligosaccharides composed of neutral and acidic components are mannose-containing oligosugars of the complex type. Their abundance is much higher in dermatosparactic p-N-collagen type III.

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