Timing and rates of synthesis of early histone mRNA in the embryo of Strongylocentrotus purpuratus

Abstract

The level of early histone mRNA in Strongylocentrotus purpuratus changes abruptly at 6 hr of development, increasing an average of 10-fold by 9-10.5 hr and then decreasing over 2-fold by 13.5-15 hr. These changes occur when the late embryonic mRNA is still a very minor component of histone gene transcription. The exact values of increase and decrease of mRNA level vary from experiment to experiment and may reflect the conditions of embryos at different times. The instantaneous rate of synthesis of histone RNA per embryo increases from at least 47 fg/min at 6 hr to 114 fg/min at 9 hr and then drops to 29 fg/min at 12 hr. The rate of mRNA accumulation is lower: 20, 43, and 12 fg/min, respectively. On a per cell basis, however, the rate of synthesis and accumulation is highest at 6 hr and continuously decreases to 1/20 the level per cell at 12 hr. The transcriptional rates and relative mRNA increases taken together predict an average increase from 0.16 to 0.24 pg/embryo (6-10 X 10(5) molecules) per mRNA species in the egg to 1.6 to 2.4 pg/embryo (6-10 X 10(6) molecules) at 10.5 hr. The transcription rates indicate that at the maximal values we obtained, about two to three molecules of each histone RNA are made per gene copy per minute. The half-life of the histone mRNAs in the period from 6 to 13.5 hr probably varies, with the maximal turnover at about the time histone RNA level peaks. A half-life of 1.5 hr at 12 hr of development is estimated. Change in transcriptional rate per nucleus, increase in cell number, and probably a change in mRNA stability as well are therefore involved in the control of histone mRNA levels in the early embryo.