Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983;52(1):17-25.
doi: 10.1007/BF00230585.

The NADPH oxidase of guinea pig polymorphonuclear leucocytes. Properties of the deoxycholate extracted enzyme

P Bellavite, M C Serra, A Davoli, J V Bannister, F Rossi

The NADPH oxidase of guinea pig polymorphonuclear leucocytes. Properties of the deoxycholate extracted enzyme

P Bellavite et al. Mol Cell Biochem. 1983.

Abstract

NADPH oxidase from stimulated guinea pig granulocytes was extracted with deoxycholate. The solubilized enzyme was stable in 20% glycerol. Solubilized enzyme was free of myeloperoxidase activity. The properties of the deoxycholate solubilized enzyme indicated that it is a high molecular weight complex with a flavoprotein, calmodulin and cytochrome b possibly forming part of the complex. Maximum activity was between pH 7.0 and 7.5. The Km value was 15.8 microM for NADPH and 434 microM for NADH indicating that NADPH is the preferential substrate.

PubMed Disclaimer

References

    1. Biochem Biophys Res Commun. 1981 Mar 31;99(2):484-90 - PubMed
    1. J Biol Chem. 1981 Mar 10;256(5):2321-3 - PubMed
    1. J Clin Invest. 1977 Feb;59(2):282-90 - PubMed
    1. J Pathol. 1982 Mar;136(3):241-52 - PubMed
    1. Nature. 1978 Nov 30;276(5687):515-7 - PubMed

Publication types

LinkOut - more resources