Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Jun 25;11(12):3889-901.
doi: 10.1093/nar/11.12.3889.

Size fractionation of Trypanosoma brucei DNA: localization of the 177-bp repeat satellite DNA and a variant surface glycoprotein gene in a mini-chromosomal DNA fraction

P Sloof, H H Menke, M P Caspers, P Borst
Free PMC article

Size fractionation of Trypanosoma brucei DNA: localization of the 177-bp repeat satellite DNA and a variant surface glycoprotein gene in a mini-chromosomal DNA fraction

P Sloof et al. Nucleic Acids Res. .
Free PMC article

Abstract

We have size-fractionated intact DNA from Trypanosoma brucei into a major large DNA fraction (greater than 350S) and minor middle-sized (60-250S) and small (less than 60S) DNA fractions. Large DNA contains the rRNA genes, the basic copy genes for several variant surface glycoproteins (VSGs), including one which lies near a telomer, and the expression-linked copies of the two VSG genes. The middle-sized DNA contains at least one VSG gene, but the hybridization of this fraction with probes for the conserved repetitive sequences that mark the edges of the transposed segments of VSG genes, suggests that it may contain many VSG genes. The 177-bp repeat satellite DNA is also exclusively found in this fraction.

PubMed Disclaimer

References

    1. J Cell Biol. 1978 Feb;76(2):293-309 - PubMed
    1. J Mol Biol. 1969 Aug 28;44(1):143-60 - PubMed
    1. J Cell Sci. 1970 Mar;6(2):365-83 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1974;38:609-18 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed

Publication types