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. 1983 Jun 25;11(12):4185-93.
doi: 10.1093/nar/11.12.4185.

The incorporation of O6-methyldeoxyguanosine and O4-methyldeoxythymidine monophosphates into DNA by DNA polymerases I and alpha

J A Hall, R Saffhill
Free PMC article

The incorporation of O6-methyldeoxyguanosine and O4-methyldeoxythymidine monophosphates into DNA by DNA polymerases I and alpha

J A Hall et al. Nucleic Acids Res. .
Free PMC article

Abstract

The modified nucleoside 5'-triphosphates O6-MedGTP ad O4-MedTTP have been synthesised and their acceptability as DNA-precursors investigated using DNA polymerases I and alpha in an in vitro assay. O6-MedGMP is only incorporated into newly-synthesized DNA-like material in the presence of templates containing thymine bases. Similarly O4-MedTMP is only incorporated in the presence of templates containing guanine bases. The results confirm the promutagenic nature and base-pairing properties of O6-MeG ad O4-MeT.

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References

    1. Nature. 1969 Jul 12;223(5202):206-7 - PubMed
    1. Biochemistry. 1970 Oct 13;9(21):4141-53 - PubMed
    1. Biochim Biophys Acta. 1973 May 10;308(2):310-6 - PubMed
    1. Biochem J. 1973 Oct;136(2):387-93 - PubMed
    1. Biochem J. 1973 Sep;135(1):193-201 - PubMed

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