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. 1983 Apr 15;130(2):471-4.
doi: 10.1016/0003-2697(83)90618-8.

Gel-exclusion chromatography on S1000 Sephacryl: application to phospholipid vesicles

J A Reynolds, Y Nozaki, C Tanford

Gel-exclusion chromatography on S1000 Sephacryl: application to phospholipid vesicles

J A Reynolds et al. Anal Biochem. .

Abstract

Sephacryl S1000 has an exclusion diameter of approximately 3000 A and is thus an appropriate gel exclusion medium for size analysis and fractionation of phospholipid vesicles. Calibration is conveniently carried out using polystyrene beads of known diameter eluted with a detergent-containing buffer. Recovery of phospholipid vesicles of different sizes from S1000 presaturated with lipid is greater than 95%, and diameters obtained from a calibration curve using the polystyrene beads are in good agreement with those obtained by negative contrast electron microscopy.

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