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. 1983 Jul 1;224(1):127-33.
doi: 10.1016/0003-9861(83)90196-0.

The carbohydrate of human thrombin: structural analysis of glycoprotein oligosaccharides by mass spectrometry

B Nilsson, M K Horne 3rd, H R Gralnick

The carbohydrate of human thrombin: structural analysis of glycoprotein oligosaccharides by mass spectrometry

B Nilsson et al. Arch Biochem Biophys. .

Abstract

The carbohydrate structure of human thrombin has been determined by direct probe mass spectrometry of the oligosaccharides released by trifluoroacetolysis from the asialo glycoprotein. The free oligosaccharides were studied as permethylated and N-trifluoroacetylated oligosaccharide alditols. The structure was confirmed by sequential exoglycosidase digestion of intact thrombin and sugar and methylation analysis of the oligosaccharides by gas-liquid chromatography-mass spectrometry. The results indicate the following structure: (formula; see text) with Fuc present on only about 50% of the oligosaccharides.

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