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. 1983 Apr 1;211(1):191-8.
doi: 10.1042/bj2110191.

Synthesis of hyaluronate in differentiated teratocarcinoma cells. Mechanism of chain growth

P Prehm

Synthesis of hyaluronate in differentiated teratocarcinoma cells. Mechanism of chain growth

P Prehm. Biochem J. .

Abstract

Hyaluronate could be labelled in vivo with [32P]phosphate. [32P]UDP in an alpha-glycosidic linkage constituted the reducing end of membrane-bound hyaluronate. The UDP is liberated during further chain elongation, indicating that chain growth occurs at the reducing end. [3H]Uridine could be incorporated into hyaluronate during synthesis on the isolated membraneous fraction from [3H]UDP-GlcNAc and [3H]UDP-GlcA, confirming the identification of UDP as a constituent of membrane-bound hyaluronate. These results led to a model of hyaluronate chain elongation at the reducing end by alternate addition of the chains to the substrates. Membrane-bound pyrophosphatases or 5'-nucleotidase are suggested as modulators of hyaluronate synthesis.

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References

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