Interneurons mediating fast postsynaptic inhibition in pyriform cortex of the rabbit

Abstract

Interneurons mediating the fast IPSPs in principal cells in the pyriform cortex (PC) of the rabbit were searched for using criteria derived from the analysis of the properties of the fast inhibitory postsynaptic potentials (IPSPs). Thirty units were identified as inhibitory interneurons. The interneurons were activated synaptically by volleys from the olfactory bulb (OB), the lateral olfactory tract (LOT), the anterior commissure (AC), and deep-lying structures of the PC (DPC). The interneurons showed a tendency to discharge repetitively in response to shocks applied to these structures of the basal forebrain (OB, LOT, AC, and DPC). The conditioning OB shocks eliminated the testing LOT-evoked discharges of the interneurons. The conditioning OB shocks eliminated the initial part of the testing OB-evoked discharges, leaving the later part relatively unchanged. On the other hand, the conditioning OB shocks did not completely eliminate the testing DPC- or AC-evoked discharges. A temporal facilitation of discharges in the interneurons was observed in response to volleys from the OB, DPC, or AC. A spatial facilitation of discharges in the interneurons was observed in response to a combination of shocks applied to the OB, DPC, and AC. The interneurons were recorded at depths 525-2,755 microns deep to the turnover point of the component 2 wave of field potentials evoked by volleys through the LOT fibers. They were located mostly in the deeper part of layer III of the PC. Intracellular recordings from the presumed inhibitory interneurons showed that OB stimulation elicited two successive excitatory postsynaptic potentials (EPSPs) on which the bursting discharges were superimposed. These EPSPs were followed by a long-lasting hyperpolarizing potential. A comparison of the latencies of the antidromic activation of the principal cells and the synaptic activation of the inhibitory interneurons following OB or DPC stimulation suggested that the inhibitory interneurons are activated at least partly through the axon collaterals of the principal cells, which project their main axons to the OB or DPC. A circuit diagram was proposed for the neuronal pathways responsible for the fast IPSPs of principal cells in the PC.