Human beta and delta globin messenger RNAs turn over at different rates

Abstract

We have tested the hypothesis of Winslow & Ingram (1966) that the steady-state levels of human beta and delta globin proteins are determined, in part, by the stabilities of their respective messenger RNAs. Nucleated bone marrow cells were cultured under different RNA "chase" conditions, and samples were harvested at intervals thereafter. The quantities of beta and delta globin mRNA sequences in total RNA from these cells were measured by solution hybridization with specific 32P-labeled DNA probes. The average half-lives of beta and delta globin mRNAs are 16.5 and 4.5 hours, respectively. Both mRNAs are polyadenylated. The rapid turnover of delta globin mRNA accounts, at least in part, for the low level of delta globin mRNA in non-nucleated peripheral blood reticulocytes. The possibility that the rate of mRNA decay is determined by nucleotide sequence signals located in the 3' untranslated region is discussed.