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. 1978 Jul;56(7):760-4.
doi: 10.1139/o78-114.

Identification of the 5S RNA binding site in intermolecular complexes of wheat embryo ribosomal 5S and 18S RNA

J L Nichols, W Wijesinghe

Identification of the 5S RNA binding site in intermolecular complexes of wheat embryo ribosomal 5S and 18S RNA

J L Nichols et al. Can J Biochem. 1978 Jul.

Abstract

The nucleotide sequence in wheat embryo ribosomal 5S RNA that binds by complementary base pairing to ribosomal 18S RNA has been identified. The 5S--18S RNA complex was formed in vitro and the interacting regions of the two RNA chains were isolated by a combination of nuclease digestion and electrophoresis on polyacrylamide gels. The binding site is close to the 3'-end of the 5S RNA molecule and contains a maximum of 24 nucleotides.

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