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. 1983 Jun;45(6):1865-9.
doi: 10.1128/aem.45.6.1865-1869.1983.

Genetic recombination in Actinoplanes brasiliensis by protoplast fusion

N J Palleroni

Genetic recombination in Actinoplanes brasiliensis by protoplast fusion

N J Palleroni. Appl Environ Microbiol. 1983 Jun.

Abstract

Protoplast formation, fusion, and cell regeneration have been achieved with mutant strains of Actinoplanes brasiliensis. Three-, four-, and five-factor crosses have shown genetic recombination among the markers, and a five-factor cross is analyzed and discussed. Possibilities of using protoplast fusion for gene mapping and strain improvement are suggested.

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