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. 1983 May 15;212(2):259-64.
doi: 10.1042/bj2120259.

Measurement of specific radioactivities in labelled hormones by self-displacement analysis

J C Calvo, J P Radicella, E H Charreau

Measurement of specific radioactivities in labelled hormones by self-displacement analysis

J C Calvo et al. Biochem J. .

Abstract

A graphical method is described that allows the determination of specific radioactivities of radioactively labelled hormones. This method combines the self-displacement technique, plotting bound/free ratios versus mass of unlabelled hormone or total radioactivity of labelled preparation added to the receptor preparation, and the maximal binding capacity of the labelled hormone. The procedure presented herein provides a more realistic specific radioactivity for use in all binding experiments. Application of the method is demonstrated for 125I-labelled ovine prolactin, and data are presented for 125I-labelled human choriogonadotropin and [3H]testosterone.

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References

    1. Biochim Biophys Acta. 1971 Dec 28;251(3):363-9 - PubMed
    1. J Biol Chem. 1975 Nov 25;250(22):8818-23 - PubMed
    1. Curr Top Mol Endocrinol. 1974;1:1-30 - PubMed
    1. Am J Physiol. 1980 Mar;238(3):E293-302 - PubMed
    1. Acta Physiol Lat Am. 1981;31(1):13-24 - PubMed