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. 1980 Aug 25;8(16):3553-64.
doi: 10.1093/nar/8.16.3553.

Brain tubulin and actin cDNA sequences: isolation of recombinant plasmids

I Ginzburg, A de Baetselier, M D Walker, L Behar, H Lehrach, A M Frischauf, U Z Littauer
Free PMC article

Brain tubulin and actin cDNA sequences: isolation of recombinant plasmids

I Ginzburg et al. Nucleic Acids Res. .
Free PMC article

Abstract

Rat brain mRNA enriched for tubulin and actin sequences was used to prepare double stranded cDNA. A library of recombinant clones was constructed by inserting the dsDNA into the Pst1 site of pBR322 plasmid and transformation of E. coli chi 1776 host. Clones bearing sequences coding for tubulin and actin were identified and characterized.

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References

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