Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982 Jul 24;10(14):4259-77.
doi: 10.1093/nar/10.14.4259.

Cloning and characterisation of the abundant cytoplasmic 7S RNA from mouse cells

A Balmain, R Krumlauf, J K Vass, G D Birnie

Cloning and characterisation of the abundant cytoplasmic 7S RNA from mouse cells

A Balmain et al. Nucleic Acids Res. .

Abstract

A cDNA library has been prepared from mouse embryo small RNAs and screened for the presence of clones complementary to the highly abundant cytoplasmic 7S RNA. One clone (pA6) was selected which hybridized exclusively with 7S RNA on a Northern blot prepared from cytoplasmic RNA run on high resolution polyacrylamide/urea gels. Sequence analysis of this clone has shown that at least 65 nucleotides at the 5' end of 7S RNA are extensively homologous with the highly repeated mouse B1 family. Heterologous hybridisations between the cloned mouse 7S sequence and RNAs prepared from rat, human and chick cells have shown that the non-B1 part of the 7S RNA molecule has been highly conserved during recent eucaryotic evolution. There are multiple copies of 7S RNA genes in the genomes of mouse, human, rat and chick cells, but substantial differences exist in copy number and genomic organisation in these organisms.

PubMed Disclaimer

References

    1. Nature. 1980 Sep 11;287(5778):109-10 - PubMed
    1. Nucleic Acids Res. 1980 Sep 25;8(18):4143-54 - PubMed
    1. Nucleic Acids Res. 1980 Mar 25;8(6):1201-15 - PubMed
    1. Nucleic Acids Res. 1980 Mar 25;8(6):1273-85 - PubMed
    1. Nucleic Acids Res. 1980 Apr 11;8(7):1643-60 - PubMed

Publication types

Associated data