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. 1982 Dec 20;10(24):7947-57.
doi: 10.1093/nar/10.24.7947.

Molecular cloning and characterization of a complete DNA copy of potato spindle tuber viroid RNA

P van Wezenbeek, P Vos, J van Boom, A van Kammen

Molecular cloning and characterization of a complete DNA copy of potato spindle tuber viroid RNA

P van Wezenbeek et al. Nucleic Acids Res. .

Abstract

Double-stranded cDNA has been synthesized from RNA of a severe strain of potato spindle tuber viroid using a synthetic oligodeoxyribonucleotide as a primer. Upon cloning in bacteriophage M13mp9, two recombinant phages were selected to construct a pBR322-derived plasmid containing a complete viroid DNA copy. Elucidation of the nucleotide sequence revealed four differences with the previously established sequence of another PSTV strain, three of which were base exchanges and one a deletion.

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References

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