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. 1980 Jun 1;187(3):909-12.
doi: 10.1042/bj1870909.

Fluorimetric assays for cathepsin B and cathepsin H with methylcoumarylamide substrates

A J Barrett

Fluorimetric assays for cathepsin B and cathepsin H with methylcoumarylamide substrates

A J Barrett. Biochem J. .

Abstract

Benzyloxycarbonyl-phenylalanyl-arginine 4-methyl-7-coumarylamide was found to be an excellent substrate for the fluorimetric assay of cathepsin B, and arginine 4-methyl-7-coumarylamide for cathepsin H. Procedures were developed that are very convenient, and avoid the hazards associated with the use of naphthylamides.

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