doi: 10.1007/BF01538967.
33258 Hoechst enhancement of the photosensitivity of bromodeoxyuridine-substituted cells
- PMID: 69327
- DOI: 10.1007/BF01538967
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33258 Hoechst enhancement of the photosensitivity of bromodeoxyuridine-substituted cells
Somatic Cell Genet.
1976 May.
Abstract
Cells that have incorporated 5-bromodeoxyuridine (BrdU) into their DNA become extremely sensitive to photoinduced killing after treatment with the dye, 33258 Hoechst. Under appropriate conditions the dye increases the efficiency of killing by a factor of approximately 10(4). This enhanced photosensitivity suggests that the combination of BrdU, dye, and light can be used to select conditional lethal mutants.
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