Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 Oct;77(10):6081-5.
doi: 10.1073/pnas.77.10.6081.

Molecular cloning of a human histocompatibility antigen cDNA fragment

H L Ploegh, H T Orr, J L Strominger

Molecular cloning of a human histocompatibility antigen cDNA fragment

H L Ploegh et al. Proc Natl Acad Sci U S A. 1980 Oct.

Abstract

A clone (pHLA-1) containing HLA-specific cDNA was constructed by reverse transcription of partially purified HLA mRNA from the human lymphoblastoid cell line LKT. The identity of pHLA-1 was established by its ability to hybridize to HLA heavy chain mRNA and by nucleotide sequence analysis. The pHLA-1 cDNA insert (approximately 525 base pairs) corresponds to the COOH-terminal 46 amino acids of an HLA-A, -B, or -C antigen (15 residues from the hydrophobic region and the remainder from the COOH-termial hydrophilic region), together with a portion of the 3' untranslated region of the mRNA.

PubMed Disclaimer

References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Nucleic Acids Res. 1975 Mar;2(3):319-25 - PubMed
    1. Nature. 1976 May 20;261(5557):200-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2481-5 - PubMed
    1. N Engl J Med. 1976 Oct 7;295(15):806-13 - PubMed

Publication types

Associated data