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. 1981 Sep;78(9):5367-70.
doi: 10.1073/pnas.78.9.5367.

Increased levels of threonyl-tRNA synthetase in a borrelidin-resistant Chinese hamster ovary cell line

J S Gantt, C A Bennett, S M Arfin

Increased levels of threonyl-tRNA synthetase in a borrelidin-resistant Chinese hamster ovary cell line

J S Gantt et al. Proc Natl Acad Sci U S A. 1981 Sep.

Abstract

The growth of Chinese hamster ovary cells in medium containing reduced concentrations of threonine is inhibited by borrelidin, a macrolide antibiotic. Borrelidin-resistant clones have been isolated after ethyl methanesulfonate mutagenesis. One clone, 1C-1, has a 3-fold increased level of threonyl-tRNA synthetase [L-threonine:tRNAThr ligase (AMP-forming), EC 6.1.1.3] as determined by both activity measurements and antiserum titrations. The levels of four other aminoacyl-tRNA synthetases and of tRNAThr are the same in strain 1C-1 and in the wild-type parent. The phenotype of increased threonyl-tRNA synthetase activity is recessive to wild type in cell hybrids.

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