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. 1981 Nov;78(11):6793-7.
doi: 10.1073/pnas.78.11.6793.

Calcium-dependent interaction of S100b, troponin C, and calmodulin with an immobilized phenothiazine

D R Marshak, D M Watterson, L J Van Eldik

Calcium-dependent interaction of S100b, troponin C, and calmodulin with an immobilized phenothiazine

D R Marshak et al. Proc Natl Acad Sci U S A. 1981 Nov.

Abstract

We have purified the brain-specific protein S100b by affinity-based adsorption chromatography on phenothiazine-Sepharose conjugates and studied the interaction of this and other calcium-modulated proteins with the immobilized antipsychotic drug. Bovine brain calmodulin, rabbit skeletal muscle troponin C, and bovine brain S100b bind to phenothiazine-Sepharose in a calcium-dependent manner. These three proteins competitively inhibit the calcium-dependent binding of 125I-labeled chicken gizzard calmodulin to the immobilized drug. However, carp parvalbumin and chicken intestinal vitamin D-dependent calcium binding protein do not inhibit the phenothiazine--calmodulin interaction. These results suggest that the known amino acid sequence homology among calmodulin, troponin C, and S100b may be reflected in a similar functional domain present in these proteins but absent in parvalbumin and vitamin D-dependent protein.

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References

    1. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6458-62 - PubMed
    1. Biochemistry. 1980 Jun 24;19(13):3113-20 - PubMed
    1. Biochem Biophys Res Commun. 1979 Oct 12;90(3):1048-56 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7039-43 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6354-8 - PubMed

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