The role of prostaglandins E2 and F2 alpha on canine arterial in vitro lipid biosynthesis from 14C-acetate

Abstract

This study was designed to investigate the influence of prostaglandin on in vitro incorporation of 14C acetate into canine aortic lipid. Aortae were excised from pentobarbital-anesthetized dogs, stripped of their adventitial layer and incubated four hours in the presence of labeled substrate alone or labeled substrate plus prostaglandin. The tissue was subsequently homogenized and the lipid phase extracted. Thin layer chromatography was used to separate lipid subfractions. Incorporated 14C was measured by liquid scintillation. PGE2 (0.05-0.10 microgram/ml) significantly decreased (p less than 0.01) incorporation of 14C acetate into phospholipid. Other lipid subfractions were not affected. PGF2 alpha (0.01-0.05 microgram/ml) significantly increased (p less than 0.01) incorporation of 14C acetate into phospholipid, triglyceride and FFA. Other subfractions were not affected. Studies conducted on intimal and medial layers separately failed to alter the extent to which 14C was incorporated into these tissue layers. Tissue "blanks" performed following destruction of enzymatic activity failed to demonstrate any significant background uptake of 14C. Therefore, in vitro effect of PGE2 is to decrease aortic wall lipid synthesis from acetate, while the effect of PGF2 alpha is to increase aortic lipid synthesis from acetate.