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. 1981 Dec;78(12):7314-7.
doi: 10.1073/pnas.78.12.7314.

Human platelet-derived growth factor (PDGF): purification of PDGF-I and PDGF-II and separation of their reduced subunits

H N Antoniades

Human platelet-derived growth factor (PDGF): purification of PDGF-I and PDGF-II and separation of their reduced subunits

H N Antoniades. Proc Natl Acad Sci U S A. 1981 Dec.

Abstract

Human platelet-derived growth factor (PDGF) was purified from lysates of clinically outdated human platelets by a direct procedure that allowed the recovery of active PDGF from stained sodium dodecyl sulfate/polyacrylamide gels. This technique enabled the identification and purification to homogeneity of two active PDGF polypeptides, and with a molecular weight of about 35,000 (PDGF-I) and the other with a molecular weight of about 32,000 (PDGF-II). Reduced PDGF-I produced two inactive subunits, with molecular weights of about 15,000 and 18,000. Reduced PDGF-II also produced two inactive subunits, with molecular weights of about 15,000 and 16,000. It is possible that PDGF-II derives from proteolytic cleavage of PDGF-I.

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References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Nature. 1974 Feb 1;247(5439):279-81 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1207-10 - PubMed
    1. Exp Cell Res. 1974 Aug;87(2):297-301 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 May;74(5):1973-7 - PubMed

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