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. 1982 Feb;79(3):754-7.
doi: 10.1073/pnas.79.3.754.

In vitro reconstitution of intermediate filaments form mammalian neurofilament triplet polypeptides

R V Zackroff, W W Idler, P M Steinert, R D Goldman

In vitro reconstitution of intermediate filaments form mammalian neurofilament triplet polypeptides

R V Zackroff et al. Proc Natl Acad Sci U S A. 1982 Feb.

Abstract

Intermediate filaments (IF) were reconstituted in vitro from bovine neurofilament triplet polypeptides. Neural IF, solubilized in either low salt or 8 M urea solution, assembled into IF when returned to near-physiological solution conditions. The 68,000-dalton component of the triplet, purified to homogeneity by preparative NaDodSO4 electrophoresis, was renatured and reassembled into short (approximatley 0.05-micrometer) approximatley 10 nm-diameter filaments. These results demonstrate that the triplet polypeptides are components of neural IF and that the 68,000-dalton polypeptide is an IF structural protein.

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