Inhibition of hormone-stimulated human fat cell-lipolysis by prostaglandin E2 and its synthetic analogue sulprostrone

Abstract

The effects of prostaglandin E2 (PG E2) and of its synthetic analogue Sulprostrone on isoproterenol-stimulated human fat cell-lipolysis and adenylate cyclase activity were compared. Glycerol release and adenylate cyclase activity were depressed to a similar maximal extent by both compounds (30%-35%) with Sulprostrone being 3 - 10-times more potent than the parent compound PG E2. The results strengthen the concept of prostaglandins exerting their antilipolytic effects via inhibition of human fat cell adenylate cyclase and demonstrate that Sulprostrone is not tissue-specific in vitro.