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. 1982 Sep;79(17):5249-53.
doi: 10.1073/pnas.79.17.5249.

Calcium/phospholipid regulates phosphorylation of a Mr "87k" substrate protein in brain synaptosomes

W C Wu, S I Walaas, A C Nairn, P Greengard

Calcium/phospholipid regulates phosphorylation of a Mr "87k" substrate protein in brain synaptosomes

W C Wu et al. Proc Natl Acad Sci U S A. 1982 Sep.

Abstract

Depolarization-induced calcium influx into rat cerebral cortex synaptosomes increased the phosphorylation of several synaptosomal proteins as examined by 32Pi incorporation. A phosphopeptide mapping technique involving NaDodSO4/polyacrylamide gels has been used to show that phosphorylation of a Mr 87,000 substrate protein is stimulated by depolarization-induced calcium influx. Phosphorylation of this Mr 87,000 substrate occurred in synaptosomal cytosol and was markedly stimulated by calcium/phosphatidylserine. Calmodulin inhibited this phosphorylation reaction. This substrate for calcium/phospholipid-dependent protein kinase is enriched in and appears to be specific to neurons.

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