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Comparative Study
. 1982 Sep;79(18):5460-4.
doi: 10.1073/pnas.79.18.5460.

Gamma-irradiation activates biochemical systems: induction of nitrate reductase activity in plant callus

K N Pandey, P S Sabharwal
Comparative Study

Gamma-irradiation activates biochemical systems: induction of nitrate reductase activity in plant callus

K N Pandey et al. Proc Natl Acad Sci U S A. 1982 Sep.

Abstract

Gamma-irradiation induced high levels of nitrate reductase activity (NADH:nitrate oxidoreductase, EC 1.6.6.1) in callus of Haworthia mirabilis Haworth. Subcultures of gamma-irradiated tissues showed autonomous growth on minimal medium. We were able to mimic the effects of gamma-irradiation by inducing nitrate reductase activity in unirradiated callus with exogenous auxin and kinetin. These results revealed that induction of nitrate reductase activity by gamma-irradiation is mediated through in vitro activation of hormone synthesis in callus cells.

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