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. 1982 Dec;79(23):7132-6.
doi: 10.1073/pnas.79.23.7132.

Sequences upstream from the T-A-T-A box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter

R Hen, P Sassone-Corsi, J Corden, M P Gaub, P Chambon

Sequences upstream from the T-A-T-A box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter

R Hen et al. Proc Natl Acad Sci U S A. 1982 Dec.

Abstract

We show that sequences located upstream from the T-A-T-A box, between positions -97 and -34, are necessary for efficient in vivo transcription from the adenovirus serotype 2 major late promoter. The effect of these upstream sequences was also investigated in vitro using a whole cell or an S100 extract and circular or linear templates. With the whole cell extract, the in vivo effect of the upstream sequences was reproduced in vitro. With the S100 extract, some effect of the upstream sequences was observed with circular, but not with linear, templates.

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References

    1. Proc Natl Acad Sci U S A. 1975 May;72(5):1843-7 - PubMed
    1. J Biol Chem. 1979 Jul 10;254(13):6163-73 - PubMed
    1. Nucleic Acids Res. 1980 Jan 11;8(1):127-42 - PubMed
    1. J Mol Biol. 1979 Oct 15;134(1):143-58 - PubMed
    1. Methods Enzymol. 1980;65(1):499-560 - PubMed

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