Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Jul;6(1):76-80.
doi: 10.1128/jcm.6.1.76-80.1977.

Plaque assay and cloning of scrub typhus rickettsiae in irradiated L-929 cells

S C Oaks Jr, J V Osterman, F M Hetrick

Plaque assay and cloning of scrub typhus rickettsiae in irradiated L-929 cells

S C Oaks Jr et al. J Clin Microbiol. 1977 Jul.

Abstract

It was demonstrated that gamma-irradiated L-929 cells support plaque formation by three strains of Rickettsia tsutsugamushi and representative species of the spotted fever and typhus group rickettsiae. Sensitivity of the plaque assay for detection of viable scrub typhus rickettsiae was similar to that achieved with intraperitoneal inoculation of random-bred mice. The concentration of irradiated cells and the temperature and length of incubation were all found to affect plaque size. A technique combining terminal dilution and plaque purification was used to obtain clones of three strains of scrub typhus rickettsiae.

PubMed Disclaimer

References

    1. Infect Immun. 1973 Jul;8(1):4-7 - PubMed
    1. Appl Microbiol. 1974 Jun;27(6):1157-61 - PubMed
    1. Infect Immun. 1972 Jul;6(1):50-7 - PubMed
    1. Infect Immun. 1972 May;5(5):715-22 - PubMed
    1. Infect Immun. 1972 Oct;6(4):550-6 - PubMed

MeSH terms

LinkOut - more resources