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. 1980 Oct 1;152(4):1036-47.
doi: 10.1084/jem.152.4.1036.

Growth of factor-dependent hemopoietic precursor cell lines

T M Dexter, J Garland, D Scott, E Scolnick, D Metcalf

Growth of factor-dependent hemopoietic precursor cell lines

T M Dexter et al. J Exp Med. .

Abstract

Cell lines have been produced from long-term cultures of mouse bone marrow that require a factor, present in WEHI-3 conditioned medium (CM) or in spleen CM, for their sustained growth. The cell lines were obtained from nonvirus-treated cultures, are nonleukemic, maintain a normal karyotype, and form colonies showing granulocyte maturation when plated in soft agar. Granulocyte/macrophage (GM) colony-stimulating factor is not the inductive moiety involved in the maintenance of proliferation of these cells. It is suggested that the cell lines represent a self-renewing population of cells ancestral to GM colony-forming cells, which may be responding to a hitherto unrecognized regulator.

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