Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1980 Sep 1;189(3):461-6.
doi: 10.1042/bj1890461.

Indoleamine 2,3-dioxygenase. A new, rapid, sensitive radiometric assay and its application to the study of the enzyme in rat tissues

J S Cook, C I Pogson, S A Smith
Comparative Study

Indoleamine 2,3-dioxygenase. A new, rapid, sensitive radiometric assay and its application to the study of the enzyme in rat tissues

J S Cook et al. Biochem J. .

Abstract

A simple and convenient assay for indoleamine 2,3-dioxygenase has been developed. This depends on the conversion of D-[ring-2-14C]tryptophan to [14C]formate, excess substrate is removed by adsorption onto charcoal. This assay, which is 20-fold more sensitive than previous procedures, is applicable both to crude extracts and to large numbers of samples. Activity in rat tissues is very much lower than in those of the rabbit; measureable activity is found only in the stomach, spleen, intestine and kidney. Enzyme activity in the rat intestine was increased by 50% in rats pretreated with L-tryptophan.

PubMed Disclaimer

References

    1. Arch Biochem Biophys. 1967 May;120(2):397-403 - PubMed
    1. J Biol Chem. 1967 Nov 25;242(22):5260-6 - PubMed
    1. Arch Biochem Biophys. 1968 Dec;128(3):637-45 - PubMed
    1. Life Sci II. 1971 Apr 22;10(8):431-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2850-2 - PubMed

Publication types