doi: 10.1128/JVI.23.1.117-125.1977.
DNA polymerase activities in growing cells infected with simian virus 40
- PMID: 69720
- PMCID: PMC515807
- DOI: 10.1128/JVI.23.1.117-125.1977
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DNA polymerase activities in growing cells infected with simian virus 40
J Virol.
1977 Jul.
Abstract
Growing CV1 cells were infected with simian virus 40 (SV40), and the levels of DNA polymerases-alpha, -beta, and -gamma were analyzed in the cytoplasm, nuclear Triton wash, and nucleus. In the cytoplasmic fraction, the amount of alpha-, beta-, or gamma-polymerase remained unaltered after SV40 infection. The activity of DNA polymerase-alpha increased five- to sixfold in the nuclear Triton wash and threefold in the nuclei and then remained enhanced only inside the nuclei. That of DNA polymerases-beta and gamma increased mostly in the nuclei after infection. These results suggest that DNA polymerase-alpha could be the major enzyme involved in SV40 DNA replication.
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