T lymphocyte-dependent B lymphocyte proliferative response to antigen. I Genetic restriction of the stimulation of B lymphocyte proliferation
- PMID: 6972992
- PMCID: PMC2186122
- DOI: 10.1084/jem.153.4.871
T lymphocyte-dependent B lymphocyte proliferative response to antigen. I Genetic restriction of the stimulation of B lymphocyte proliferation
Abstract
For the purpose of examining more closely the interaction between T and B lymphocytes, we have developed an in vitro T lymphocyte-dependent B lymphocyte proliferation assay. Proliferation of B lymphocytes in response to antigen was found to depend on the presence of primed T lymphocytes; the B lymphocytes could be derived from nonprimed animals. It appears that these B cells were nonspecifically recruited to proliferate. This nonspecific recruitment, however, was found to be Ir-gene restricted in that B lymphocytes from B10.S mice, which are genetic nonresponders to the polymer Glu60-Ala30-Tyr10 (GAT), could not be stimulated by GAT-primed (responder X nonresponder) F1 T cells. The apparent lack of antigen specificity in the face of Ir gene-restricted T-B interaction may have important implications in our understanding of the recognition unit(s) on T lymphocytes.
Similar articles
-
B lymphocyte immune response gene phenotype is genetically determined.J Exp Med. 1982 Apr 1;155(4):1239-44. doi: 10.1084/jem.155.4.1239. J Exp Med. 1982. PMID: 6801187 Free PMC article.
-
T lymphocyte-dependent B lymphocyte proliferative response to antigen. II. Induction of polyclonal B lymphocyte activation of normal B cells and of Lyb-5- B cells from xid mice via recognition of self-IA antigens.J Immunol. 1984 Oct;133(4):1792-800. J Immunol. 1984. PMID: 6206138
-
Adaptive differentiation of murine lymphocytes. IV (Responder x nonresponder) F1 T cells can be taught to preferentially help nonresponder, rather than responder, B cells.J Exp Med. 1979 Jul 1;150(1):20-30. doi: 10.1084/jem.150.1.20. J Exp Med. 1979. PMID: 109573 Free PMC article.
-
Antigen-specific T cell clones restricted to unique F1 major histocompatibility complex determinants. Inhibition of proliferation with monoclonal anti-Ia antibody.J Exp Med. 1981 Mar 1;153(3):677-93. doi: 10.1084/jem.153.3.677. J Exp Med. 1981. PMID: 6166704 Free PMC article.
-
Epitope-specific and idiotype-specific cellular interactions in a model protein antigen system.Springer Semin Immunopathol. 1980 Aug;3(2):145-70. doi: 10.1007/BF02053974. Springer Semin Immunopathol. 1980. PMID: 6168023 Review. No abstract available.
Cited by
-
The murine bm12 gene conversion provides evidence that T cells recognize predominantly Ia conformation.Proc Natl Acad Sci U S A. 1985 Oct;82(20):7058-62. doi: 10.1073/pnas.82.20.7058. Proc Natl Acad Sci U S A. 1985. PMID: 3931082 Free PMC article.
-
Activation of antigen-specific B cells: role of T cells, cytokines, and antigen in induction of growth and differentiation.Proc Natl Acad Sci U S A. 1983 Nov;80(21):6628-31. doi: 10.1073/pnas.80.21.6628. Proc Natl Acad Sci U S A. 1983. PMID: 6605533 Free PMC article.
-
Method for identifying microbial antigens that stimulate specific lymphocyte responses: application to Salmonella.Proc Natl Acad Sci U S A. 1990 Dec;87(24):9823-7. doi: 10.1073/pnas.87.24.9823. Proc Natl Acad Sci U S A. 1990. PMID: 2148211 Free PMC article.
-
Development of specific and cross-reactive lymphocyte proliferative responses during chronic immunizing infections with Rickettsia tsutsugamushi.Infect Immun. 1983 Apr;40(1):147-56. doi: 10.1128/iai.40.1.147-156.1983. Infect Immun. 1983. PMID: 6187688 Free PMC article.
-
Antigen-specific helper T-cell clone supernatant is sufficient to induce both polyclonal proliferation and differentiation of small resting B lymphocytes.Proc Natl Acad Sci U S A. 1984 Oct;81(20):6491-5. doi: 10.1073/pnas.81.20.6491. Proc Natl Acad Sci U S A. 1984. PMID: 6238333 Free PMC article.
