T-Cell hydrids: I. Selection of thymidine kinase deficient subline EL-4R from EL-4 cell population

Abstract

Thymidine kinase deficient (TK-) cell line EL-4R suitable for production of T-cell hybrids has been selected from the EL-4 cell population by treatment with 5-bromodeoxyuridine. Comparison of the EL-4 and EL-4R cell lines revealed differences in chromosome constitution and electrophoretic mobility of both cell populations. Both, EL-4 and EL-4R cells had a modal number of 39 chromosomes; the percentage of cells with modal number of chromosomes and the percentage of biarmed chromosomes in the EL-4R line was higher than that of the EL-4 line. Anodic electrophoretic mobility of EL-4R cell population was faster than that of EL-4 cells, respective mean values being 0.96 and 0.91 X 10(-4) cm2 s-1 V-1.