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. 1981;44(2):205-9.
doi: 10.1016/0022-1759(81)90348-3.

Optimal culture conditions for in vitro antigen-induced proliferation of rat lymph node cells

P G Holt, S Leivers, L A Warner

Optimal culture conditions for in vitro antigen-induced proliferation of rat lymph node cells

P G Holt et al. J Immunol Methods. 1981.

Abstract

Popliteal and inguinal lymph node cells (LNC) from rats immunized with ovalbumin (OA) in Freund's adjuvant, were cultured with OA for 4 days in a variety of serum-supplemented media. Incorporation of [3H]thymidine into [3H]DNA was assessed as an index of antigen-induced lymphoproliferation. Optimal conditions for proliferation were found to comprise culture in flat-bottomed vessels, in the presence of RPMI 1640 medium adjusted to rat osmolality (310 mosmoles/kg) containing 5% v/v fresh syngeneic rat serum plus 4 X 10(-4) M 2-mercaptoethanol.

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