Altered activities of hepatic and extrahepatic microsomal mixed function oxidase enzymes in diabetic and adrenalectomized diabetic rats

Abstract

Microsomal aryl hydrocarbon hydroxylase (AHH) and 7-ethoxycoumarin-O-deethylase (ECD) activities were determined in liver, lung and intestine of control, streptozotocin(STZ)-diabetic, and diabetic-adrenalectomized male and female rats. Hepatic cytochrome P-450 content was also determined. The diabetic state reduced hepatic AHH activity and increased ECD activity in control male rats, and failed to do so in the adrenalectomized male rats. The diabetic state increased hepatic AHH and ECD activities in the control female rats, while in the adrenalectomized female rats the activities of both enzymes were decreased compared to the control diabetic rats. STZ-induced diabetes produced a decrease in pulmonary AHH and ECD activities and increased intestinal AHH and ECD activities in both sexes. The diabetic state in the adrenalectomized rats resulted in further reduction in pulmonary AHH and ECD activities in both sexes, but failed to increase the intestinal AHH activity only in the female rat. Hepatic cytochrome P-450 contents were increased in the female but not male adrenalectomized rats when treated with STZ. The effects of STZ-induced diabetes in adrenalectomized rats on AHH, ECD and hepatic cytochrome P-450 content in liver, lung and intestine depended upon sex of animal, substrate and tissue.