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. 1978 Jul 15;174(1):153-161.
doi: 10.1042/bj1740153.

A method for the analysis of protein turnover characteristics. Indirect estimation of rates of protein degradation

N Paskin, R J Mayer

A method for the analysis of protein turnover characteristics. Indirect estimation of rates of protein degradation

N Paskin et al. Biochem J. .

Abstract

A method for the calculation of rate constants of degradation of a specific protein during a period of change in protein amount is described. The calculation uses measurements of rates of synthesis and amount of the protein, together with one estimate of the protein half-life. The method is demonstrated by using data from measurements made on the accumulation of fatty acid synthetase in hormonally treated explants of mammary gland from mid-pregnant rabbits, and data from other authors. These calculations demonstrate a regulated programme of co-ordinated changes in rates of synthesis and degradation during a period of change in protein mass. The role of such changes in protein degradation during protein accumulation is discussed.

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