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. 1982 Apr;21(4):688-90.
doi: 10.1128/AAC.21.4.688.

Variables in demonstrating methicillin tolerance in Staphylococcus aureus strains

K Ishida, P A Guze, G M Kalmanson, K Albrandt, L B Guze

Variables in demonstrating methicillin tolerance in Staphylococcus aureus strains

K Ishida et al. Antimicrob Agents Chemother. 1982 Apr.

Abstract

Certain technical considerations which affected the status of methicillin tolerance in Staphylococcus aureus strains were studied. Methods which consistently demonstrated tolerance or intolerance of a given strain were avoidance of inoculum splashing, use of stationary-phase inoculum, 24-h tube incubation, and minimization of antibiotic carry-over. These studies suggested a need for the establishment of a standardized reference for the determination of tolerance.

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